Food and Drug Administration

Center for Food Safety and Applied Nutrition (CFSAN, Zentrum für Lebensmittelsicherheit und angewandte Ernährung)

FDA, Division of Microbiology, Center for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, College Park, MD 20740, USA


Genome sequencing and comparative genomics of enterohemorrhagic Escherichia coli O145:H25 and O145:H28 reveal distinct evolutionary paths and marked variations in traits associated with virulence & colonization. SC Lorenz, N. Gonzalez-Escalona, ML Kotewicz, M. Fischer and JA Kase. BMC Microbiol. 2017 Aug 22;17(1):183. doi: 10.1186/s12866-017-1094-3 (2017)

Plasmids from Shiga toxin-producing Escherichia coli of rare Enterohemolysin (ehxA) Subtypes reveal Pathogenicity Potential and display a Novel Evolutionary Path
Sandra C. Lorenz, Steven R. Monday, Maria Hoffmann, Julie A. Kase, Fischer Markus
Applied and Environmental Microbiology 82, 6367-6377 (2016)

Complete Genome Sequences of Four Enterohemolysin-Positive (ehxA) Enterocyte Effacement-Negative Shiga Toxin-Producing Escherichia coli Strains
Sandra C. Lorenz, Michael L. Kotewicz, Maria Hoffmann, Narjol Gonzalez-Escalona, Markus Fischer, Julie A. Kase
Genome Announcements 4, 5 (2016)

Improved PCR-RFLP Method for the Identification of Escherichia coli Enterohemolysin (ehxA) Subtypes
Sandra C. Lorenz, Markus Fischer and Julie A. Kase
Journal of Microbiological Methods (accepted) (2014)

Prevalence of Hemolysin Genes and Comparison of ehxA Subtype Patterns in Shiga toxin-producing Escherichia coli (STEC) and non-STEC Strains from Clinical, Food, and Animal Sources
Sandra C. Lorenz, Insook Son, Anna Maounounen-Laasri, Andrew Lin, Markus Fischer, Julie A. Kase
Applied and Environmental Microbiology Published ahead of print 9 August 2013 (2013)

Genetic Characterization of Escherichia coli O104 Isolates from Different Sources in the United States.
Rump LV, Bodeis-Jones S, Abbott J, Zhao S, Kase J, Lorenz S, Fischer M, Brown E, Meng J.
Applied and Environmental Microbiology 78, 1615-1618 (2012)

Genetic and phylogenetic evidence for misidentification of Vibrio species within the Harveyi clade.
Maria Hoffmann; Steven R. Monday; Markus Fischer; Eric W. Brown
Letters in Applied Microbiology 54, 160-165 (2012)
Applied and Environmental Microbiology 78, 7120-7120 (2012)

Vibrio caribbeanicus sp. nov., isolated from marine sponge Scleritoderma cyanea
Maria Hoffmann; Steven R. Monday; Marc W. Allard; Errol A. Strain; Paul Whittaker; Marianna Naum; Peter J. McCarthy; Jose V. Lopez; Markus Fischer; Eric W. Brown
International Journal of Systematic and Evolutionary Microbiology in press (2011)

Different IS629 Transposition Frequencies Exhibited by Escherichia coli O157:H7 Strains in the Stepwise Evolutionary Model
Lydia V. Rump; Markus Fischer; Narjol Gonzalez-Escalona
Applied and Environmental Microbiology 77, 5030-5033 (2011)

Prevalence, distribution and evolutionary significance of the IS629 insertion element in the stepwise emergence of Escherichia coli O157:H7
Lydia V. Rump; Markus Fischer; Narjol Gonzalez-Escalona
BMC Microbiology 11, 133 (2011)

Draft genome sequences of six Escherichia coli isolates from the stepwise model of emergence of Escherichia coli O157:H7
Lydia V. Rump; E. A. Strain; G. Cao; M. W. Allard; Markus Fischer; E. W. Brown; Narjol Gonzalez-Escalona
Journal of Bacteriology 193, 2058-2059 (2011)

PCR-based method for targeting 16S-23S rRNA intergenic spacer regions among Vibrio species
Maria Hoffmann; Eric W. Brown; Peter C. H. Feng; Christine E. Keys; Markus Fischer; Steven R. Monday
BMC Microbiology 10, 90-90 (2010)

Evaluating the use of fatty acid profiles to identify deep-sea Vibrio isolates
Maria Hoffmann; Markus Fischer; Paul Whittaker
Food Chemistry 122, 943-950 (2010)

Characterization of a gne::IS629 O rough:H7 Escherichia coli strain from a hemorrhagic colitis patient
Lydia V. Rump; L. Beutin; Markus Fischer; Peter C. H. Feng
Applied and Environmental Microbiology 76, 5290-5291 (2010)

Population dynamics of Vibrio spp. associated with marine sponge microcosms
Maria Hoffmann; Markus Fischer; Andrea Ottesen; Peter J. McCarthy; Jose V. Lopez; Eric W. Brown; Stefan R. Monday
ISME Journal 1608-1612 (2010)

FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, Dauphin Island, AL 36528, USA


Examination of clinical and environmental Vibrio parahaemolyticus isolates by multi-locus sequence typing (MLST) and multiple-locus variable-number tandem-repeat analysis (MLVA)
Lüdeke CH, Gonzalez-Escalona N, Fischer M, Jones JL.
Front Microbiol. 2015 Jun 10;6:564.

Complete Genome Sequences of a Clinical Isolate and an Environmental Isolate of Vibrio parahaemolyticus.
Lüdeke CH, Kong N, Weimer BC, Fischer M, Jones JL.
Genome Announc. 2015 Mar 26;3(2). pii: e00216-15. doi: 10.1128/genomeA.00216-15.

Suitability of the molecular subtyping methods intergenic spacer region, direct genome restriction analysis, and pulsed-field gel electrophoresis for clinical and environmental Vibrio parahaemolyticus isolates.
Lüdeke CH, Fischer M, LaFon P, Cooper K, Jones JL.
Foodborne Pathog Dis. 2014 Jul;11(7):520-8. doi: 10.1089/fpd.2013.1728. Epub 2014 May 5.

Biochemical, Serological, and Virulence Characterization of Clinical and Oyster Vibrio parahaemolyticus Isolates
Jessica L. Jones, Catharina H. M. Lüdeke, John C. Bowers, Nancy Garrett, Markus Fischer, Michele B. Parsons, Cheryl A. Bopp, Angelo DePaola
Journal of Clinical Microbiology 50, 2343-2352 (2012)